Expression of Escherichia coli araE and modified lacY genes

Expression of Escherichia coli araE and modified lacY genes

Expression of Escherichia coli araE and modified lacY genes in Campylobacter jejuni will not be enough for arabinose transport

Introduction: Not like Escherichia coli , Campylobacter jejuni is unable to import a variety of sugars, together with arabinose, which makes frequent expression vectors, comparable to pBAD33, non-functional in these micro organism.

Purpose: The intention of this research was to research whether or not the E. coli transporters AraE and modified LacY (LacYA177C) would allow C. jejuni to uptake arabinose.

Methodology and outcomes: The respective genes of E. coli have been constitutively expressed in C. jejuni pressure 11168H after integration into the chromosome through homologous recombination. Vectors carrying these genes additionally contained a reporter gene, gfp, beneath the management of the arabinose-inducible promoter, pBAD. These constructs have been verified in E. coli by demonstrating the induction of gfp within the presence of arabinose. Integration of the genes into one of many rRNA gene clusters was verified by PCR and genome sequencing.

The latter additionally confirmed that the inserted gene clusters contained no mutations. Expression of the gfp gene within the presence of arabinose inducer was monitored utilizing fluorescence microscopy of colonies and fluorimetry utilizing each entire cells and lysates.

Conclusion: The outcomes demonstrated the lack of C. jejuni to make use of arabinose transporters, that are totally purposeful in E. coli , suggesting a outstanding distinction within the physiology of those micro organism.

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Anti-GCNF antibody

PAab03390 100 ug
EUR 355

anti-GCNF

YF-PA11986 50 ul
EUR 363
Description: Mouse polyclonal to GCNF

anti-GCNF

YF-PA11987 50 ug
EUR 363
Description: Mouse polyclonal to GCNF

anti-GCNF

YF-PA11988 100 ul
EUR 403
Description: Rabbit polyclonal to GCNF

anti-GCNF

YF-PA11989 100 ug
EUR 403
Description: Rabbit polyclonal to GCNF

anti-GCNF

YF-PA23769 50 ul
EUR 334
Description: Mouse polyclonal to GCNF

GCNF ELISA KIT|Human

EF009808 96 Tests
EUR 689

Germ Cell Nuclear Factor (GCNF) Antibody

20-abx131389
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Polyclonal NR6A1 / GCNF Antibody (Ligand-binding Domain)

AMM06820G 0.05mg
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NR6A1 / GCNF (Ligand-binding Domain). This antibody is tested and proven to work in the following applications:

Recombinant Germ Cell Nuclear Factor (GCNF)

4-RPC509Hu01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
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  • EUR 3544.00
  • 100 ug
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  • 1 mg
  • 200 ug
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  • 5 mg
  • Uniprot ID: Q15406
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 27.2kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Germ Cell Nuclear Factor expressed in: E.coli

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Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human)

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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF)

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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with APC.

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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with Biotin.

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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with Cy3.

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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with FITC.

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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
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Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with HRP.

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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
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  • Sequence of the immunogen: GCNF (Glu282~Glu480)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with APC-Cy7.

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Description: H2B Antibody Antibody detects endogenous levels of H2B.

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anti- Antibody^Polyclonal antibody control antibody

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Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

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STJ111369 100 µl
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Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

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STJ111530 100 µl
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Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

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STJ25475 100 µl
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Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
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Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody

STJ28365 100 µl
EUR 277

Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ114081 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

STJ114828 100 µl
EUR 277

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
EUR 277
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

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EUR 277

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STJ118752 100 µl
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Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
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Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

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Cytokeratin 7 antibody-Cytoskeleton Marker Antibody

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EUR 239

Antibody Pair to ApoA-V antibody

10R-1876 100 ul
EUR 651
Description: Mouse monoclonal Antibody Pair to ApoA-V antibody

Anti CD22 Antibody: CD22 Monoclonal Antibody

065-A-01mg 0,1 mg
EUR 267.5
  • Category: Antibody, Signal Transduction Antibodies, mAb
Description: anti-CD22 monoclonal antibody

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EUR 1282.5
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Ly1 Antibody Reactive Homolog (LYAR) Antibody

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Ly1 Antibody Reactive Homolog (LYAR) Antibody

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Monoclonal NGF/proNGF Neutralizing Antibody Antibody

AMM06679G 0.05mg
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Anti Deoxyribonucleic Acid Antibody (DNA) Antibody

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Anti-Glycoprotein Antibody (GP) Antibody (HRP)

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Anti-Glycoprotein Antibody (GP) Antibody (FITC)

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Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

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Anti-Glycoprotein Antibody (GP) Antibody (HRP)

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Anti-Glycoprotein Antibody (GP) Antibody (FITC)

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  • EUR 411.00
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

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  • EUR 411.00
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Anti-Glycoprotein Antibody (GP) Antibody (HRP)

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  • EUR 411.00
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  • EUR 411.00
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

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  • EUR 411.00
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Anti-Glycoprotein Antibody (GP) Antibody (HRP)

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  • EUR 411.00
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  • 100 ug
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  • EUR 411.00
  • EUR 1845.00
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  • 100 ug
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

20-abx319931
  • EUR 411.00
  • EUR 1845.00
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Anti-Glycoprotein 210 Antibody (gp210) Antibody

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Ly1 Antibody Reactive (LYAR) Antibody (HRP)

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Ly1 Antibody Reactive (LYAR) Antibody (FITC)

20-abx311667
  • EUR 411.00
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Ly1 Antibody Reactive (LYAR) Antibody (Biotin)

20-abx311668
  • EUR 411.00
  • EUR 1845.00
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  • 50 ug
  • Shipped within 5-10 working days.

Goat anti- human Antibody^Polyclonal antibody

LSMab09896 100 ug
EUR 438

Antibody for Human alpha Tubulin Antibody

SPC-692D 0.1mg
EUR 354
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is unconjugated.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A390 0.1mg
EUR 401
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 390.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A488 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 488.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A565 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 565.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A594 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 594.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A633 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 633.

Genome-Huge Evaluation of Peptidoglycan Recognition Protein Genes in Fig Wasps (Hymenoptera, Chalcidoidea)

  • The innate immunity is a very powerful protection in opposition to pathogen of bugs, and the peptidoglycan recognition proteins (PGRPs) play an essential function within the processes of immune recognition and initiation of Toll, IMD and different sign pathways.
  • In fig wasps, pollinators and non-pollinators current completely different evolutionary histories and existence, though each are carefully related to fig syconia, which can point out their completely different patterns within the evolution of PGRPs.
  • By handbook annotation, we bought all of the PGRP genes of 12 fig wasp species, containing seven pollinators and 5 non-pollinators, and investigated their putative completely different evolutionary patterns.
  • We discovered that the variety of PGRP genes in pollinators was considerably decrease than in non-pollinators, and the variety of catalytic PGRP introduced a declining pattern in pollinators.
  • Extra importantly, PGRP-SA is related to initiating the Toll pathway, in addition to gram-negative bacteria-binding proteins (GNBPs), which have been fully misplaced in pollinators, which led us to invest that the initiation of Toll pathway was less complicated in pollinators than in non-pollinators.
  • We concluded that fig pollinators owned a extra streamlined innate immune recognition system than non-pollinators. Our outcomes present molecular proof for the adaptive evolution of innate immunity in bugs of host specificity.

Comparative genomics of Alexander Fleming’s authentic Penicillium isolate (IMI 15378) reveals sequence divergence of penicillin synthesis genes 

Antibiotics have been derived initially from wild organisms and due to this fact understanding how these compounds evolve amongst completely different lineages would possibly assist with the design of recent antimicrobial medicine. We report the draft genome sequence of Alexander Fleming’s authentic fungal isolate behind the invention of penicillin, now labeled as Penicillium rubens Biourge (1923) (IMI 15378). We examine the construction of the genome and genes concerned in penicillin synthesis with these in two ‘excessive producing’ industrial strains of P. rubens and the carefully associated species P. nalgiovense.

The primary effector genes for producing penicillin G (pcbAB, pcbC and penDE) present amino acid divergence between the Fleming pressure and each industrial strains, whereas a set of regulatory genes are conserved. Homologs of penicillin N effector genes cefD1 and cefD2 have been additionally discovered and the latter displayed amino acid divergence between the Fleming pressure and industrial strains.

The draft assemblies include a number of partial duplications of penicillin-pathway genes in all three P. rubens strains, to differing levels, which we hypothesise could be concerned in regulation of the pathway.

The 2 industrial strains are equivalent in sequence throughout all effector and regulatory genes however differ in duplication of the pcbAB-pcbC-penDE advanced and partial duplication of fragments of regulatory genes. We conclude that evolution within the wild encompassed each sequence adjustments of the effector genes and gene duplication, whereas human-mediated adjustments by mutagenesis and synthetic choice led to duplication of the penicillin pathway genes.

Anti-ACTN3 antibody
STJ114730 100 µl
EUR 277
Description: This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Human Actinin Alpha 3 (ACTN3) ELISA Kit
DLR-ACTN3-Hu-48T 48T
EUR 498
  • Should the Human Actinin Alpha 3 (ACTN3) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Actinin Alpha 3 (ACTN3) in samples from tissue homogenates, cell lysates or other biological fluids.
Human Actinin Alpha 3 (ACTN3) ELISA Kit
DLR-ACTN3-Hu-96T 96T
EUR 647
  • Should the Human Actinin Alpha 3 (ACTN3) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Actinin Alpha 3 (ACTN3) in samples from tissue homogenates, cell lysates or other biological fluids.
Human Actinin Alpha 3 (ACTN3) ELISA Kit
RD-ACTN3-Hu-48Tests 48 Tests
EUR 500
Human Actinin Alpha 3 (ACTN3) ELISA Kit
RD-ACTN3-Hu-96Tests 96 Tests
EUR 692
Human Actinin Alpha 3 (ACTN3) ELISA Kit
RDR-ACTN3-Hu-48Tests 48 Tests
EUR 522
Human Actinin Alpha 3 (ACTN3) ELISA Kit
RDR-ACTN3-Hu-96Tests 96 Tests
EUR 724
Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349
Anti-ACTN3 Antibody (monoclonal, 9B5)
M02693 100ug/vial
EUR 334
ACTN3 Antibody
34157-100ul 100ul
EUR 252
ACTN3 Antibody
34157-50ul 50ul
EUR 187
ACTN3 Antibody
1-CSB-PA000816
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/20000
ACTN3 Antibody
1-CSB-PA001243LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200
ACTN3 Antibody
1-CSB-PA124802
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000
ACTN3 Antibody
DF8016 200ul
EUR 304
Description: ACTN3 Antibody detects endogenous levels of total ACTN3.
ACTN3 Antibody
1-CSB-PA288460
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000
ACTN3 antibody
70R-49332 100 ul
EUR 244
Description: Purified Polyclonal ACTN3 antibody
ACTN3 antibody
70R-32269 100 ug
EUR 327
Description: Rabbit polyclonal ACTN3 antibody
ACTN3 Antibody
ABD7803 100 ug
EUR 438
ACTN3 Antibody
ABD8016 100 ug
EUR 438
ACTN3 antibody
PAab10008 100 ug
EUR 386
ACTN2/ACTN3 Antibody
1-CSB-PA000815
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000
ACTN2/ACTN3 Antibody
CSB-PA960396-
EUR 335
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
  • Show more
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500
ACTN2/ACTN3 Antibody
CSB-PA960396-100ul 100ul
EUR 316
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
  • Show more
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500
Polyclonal ACTN3 Antibody
APR14796G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 . This antibody is tested and proven to work in the following applications:
ACTN3 Conjugated Antibody
C34157 100ul
EUR 397
ACTN2 / ACTN3 Antibody
20-abx329446
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
ACTN2 / ACTN3 Antibody
abx332374-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.
ACTN3 siRNA
20-abx906644
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.
ACTN3 siRNA
20-abx906645
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.
ACTN3 Antibody, HRP conjugated
1-CSB-PA001243LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
ACTN3 Antibody, FITC conjugated
1-CSB-PA001243LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
ACTN3 Antibody, Biotin conjugated
1-CSB-PA001243LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Polyclonal ACTN3 Antibody (Center)
APR14798G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 (Center). This antibody is tested and proven to work in the following applications:
Anti-Human ACTN3 DyLight® 488 conjugated Antibody
A02693-Dyl488 100ug/vial
EUR 344
Anti-Human ACTN3 DyLight® 550 conjugated Antibody
A02693-Dyl550 100ug/vial
EUR 344
Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280
Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280
Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280
ACTN3 Rabbit pAb
A12797-100ul 100 ul
EUR 308
ACTN3 Rabbit pAb
A12797-200ul 200 ul
EUR 459
ACTN3 Rabbit pAb
A12797-20ul 20 ul
EUR 183
ACTN3 Rabbit pAb
A12797-50ul 50 ul
EUR 223
ACTN3 Rabbit pAb
A12864-100ul 100 ul
EUR 308
ACTN3 Rabbit pAb
A12864-200ul 200 ul
EUR 459
ACTN3 Rabbit pAb
A12864-20ul 20 ul
EUR 183
ACTN3 Rabbit pAb
A12864-50ul 50 ul
EUR 223
ACTN3 Blocking Peptide
DF8016-BP 1mg
EUR 195
ACTN3 Blocking Peptide
20-abx062207
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
ACTN1/ACTN2/ACTN3/ACTN4 Antibody
1-CSB-PA000814
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000
ACTN1/ACTN2/ACTN3/ACTN4 Antibody
CSB-PA096347-
EUR 335
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
  • Show more
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
ACTN1/ACTN2/ACTN3/ACTN4 Antibody
CSB-PA096347-100ul 100ul
EUR 316
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
  • Show more
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Actinin Alpha 3 (ACTN3) Antibody
20-abx009624
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Actinin Alpha 3 (ACTN3) Antibody
abx026732-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.
Actinin Alpha 3 (ACTN3) Antibody
abx026732-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.
Alpha Actinin 3 (ACTN3) Antibody
20-abx213418
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.
Alpha Actinin 3 (ACTN3) Antibody
20-abx213419
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.
Actinin Alpha 3 (ACTN3) Antibody
abx038105-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Actinin Alpha 3 (ACTN3) Antibody
20-abx130639
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Actinin Alpha 3 (ACTN3) Antibody
20-abx013892
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.
Polyclonal ACTN3 Antibody (aa1-50)
APR14797G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 (aa1-50). This antibody is tested and proven to work in the following applications:
ACTN1 / ACTN2 / ACTN3 / ACTN4 Antibody
abx332562-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.
Alpha Actinin 3 (ACTN3) Antibody
20-abx328117
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
ACTN1 / ACTN2 / ACTN3 / ACTN4 Antibody
20-abx328684
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Actinin Alpha 3 (ACTN3) Antibody
abx230122-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.
Human ACTN3 ELISA Kit
EHA0390 96Tests
EUR 521
Human ACTN3 ELISA Kit
ELA-E1104h 96 Tests
EUR 824
Goat ACTN3 ELISA Kit
EGTA0390 96Tests
EUR 521
Canine ACTN3 ELISA Kit
ECA0390 96Tests
EUR 521
Anserini ACTN3 ELISA Kit
EAA0390 96Tests
EUR 521
Bovine ACTN3 ELISA Kit
EBA0390 96Tests
EUR 521
ACTN3 ELISA KIT|Human
EF003149 96 Tests
EUR 689
Human ACTN3 shRNA Plasmid
20-abx950055
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.
Mouse ACTN3 shRNA Plasmid
20-abx969019
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.
Mouse ACTN3 ELISA Kit
EMA0390 96Tests
EUR 521
Rat ACTN3 ELISA Kit
ERA0390 96Tests
EUR 521
Rabbit ACTN3 ELISA Kit
ERTA0390 96Tests
EUR 521
Porcine ACTN3 ELISA Kit
EPA0390 96Tests
EUR 521
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human)
4-PAB104Hu01
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: ACTN3 (Glu162~Lys416)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3)
Guinea Pig ACTN3 ELISA Kit
EGA0390 96Tests
EUR 521
Actn3 ORF Vector (Rat) (pORF)
ORF063016 1.0 ug DNA
EUR 506
ACTN3 ORF Vector (Human) (pORF)
ORF012141 1.0 ug DNA
EUR 354
Actn3 ORF Vector (Mouse) (pORF)
ORF038023 1.0 ug DNA
EUR 506
Recombinant Actinin Alpha 3 (ACTN3)
4-RPB104Hu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q08043
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 33.8kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Actinin Alpha 3 expressed in: E.coli
ACTN3 ELISA Kit (Mouse) (OKEH05687)
OKEH05687 96 Wells
EUR 662
Description: Description of target: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 41 pg/mL
ACTN3 ELISA Kit (Human) (OKEH04449)
OKEH04449 96 Wells
EUR 662
Description: Description of target: This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 4.1 pg/mL
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), APC
4-PAB104Hu01-APC
  • EUR 333.00
  • EUR 3113.00
  • EUR 872.00
  • EUR 423.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: ACTN3 (Glu162~Lys416)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with APC.
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), Biotinylated
4-PAB104Hu01-Biotin
  • EUR 303.00
  • EUR 2341.00
  • EUR 697.00
  • EUR 369.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: ACTN3 (Glu162~Lys416)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with Biotin.
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), Cy3
4-PAB104Hu01-Cy3
  • EUR 403.00
  • EUR 4109.00
  • EUR 1121.00
  • EUR 523.00
  • EUR 245.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: ACTN3 (Glu162~Lys416)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with Cy3.
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), FITC
4-PAB104Hu01-FITC
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: ACTN3 (Glu162~Lys416)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with FITC.
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), HRP
4-PAB104Hu01-HRP
  • EUR 305.00
  • EUR 2714.00
  • EUR 772.00
  • EUR 383.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: ACTN3 (Glu162~Lys416)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with HRP.
Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), PE
4-PAB104Hu01-PE
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: ACTN3 (Glu162~Lys416)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with PE.
Human Actinin Alpha 3 (ACTN3) Protein
20-abx167185
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Actinin Alpha 3 (ACTN3) ELISA Kit
20-abx156912
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.
Actinin Alpha 3 (ACTN3) CLIA Kit
20-abx492418
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

Transposon Insertion within the purL Gene Induces Biofilm Depletion in Escherichia coli ATCC 25922

Present Escherichia coli antibiofilm therapies comprise a mix of antibiotics generally used in opposition to planktonic cells, resulting in therapy failure. A greater understanding of the genes concerned in biofilm formation may facilitate the event of environment friendly and particular new antibiofilm therapies.

A complete of 2578 E. coli mutants have been generated by transposon insertion, of which 536 have been analysed on this research. After sequencing, Tn263 mutant, labeled as low biofilm-former (LF) in contrast to the wild-type (wt) pressure (ATCC 25922), confirmed an interruption within the purL gene, concerned within the de novo purine biosynthesis pathway.

To elucidate the function of purL in biofilm formation, a knockout was generated displaying decreased manufacturing of curli fibres, resulting in an impaired biofilm formation. These situations have been restored by complementation of the pressure or addition of exogenous inosine. Proteomic and transcriptional analyses have been carried out to characterise the variations attributable to purL alterations.

13 proteins have been altered in contrast to wt. The corresponding genes have been analysed by qRT-PCR not solely within the Tn263 and wt, but additionally in medical strains with completely different biofilm exercise. Total, this research means that purL is important for biofilm formation in E. coli and might be thought of as a possible antibiofilm goal.

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