Expression of Escherichia coli araE and modified lacY genes

Expression of Escherichia coli araE and modified lacY genes

Expression of Escherichia coli araE and modified lacY genes in Campylobacter jejuni will not be enough for arabinose transport

Introduction: Not like Escherichia coli , Campylobacter jejuni is unable to import a variety of sugars, together with arabinose, which makes frequent expression vectors, comparable to pBAD33, non-functional in these micro organism.

Purpose: The intention of this research was to research whether or not the E. coli transporters AraE and modified LacY (LacYA177C) would allow C. jejuni to uptake arabinose.

Methodology and outcomes: The respective genes of E. coli have been constitutively expressed in C. jejuni pressure 11168H after integration into the chromosome through homologous recombination. Vectors carrying these genes additionally contained a reporter gene, gfp, beneath the management of the arabinose-inducible promoter, pBAD. These constructs have been verified in E. coli by demonstrating the induction of gfp within the presence of arabinose. Integration of the genes into one of many rRNA gene clusters was verified by PCR and genome sequencing.

The latter additionally confirmed that the inserted gene clusters contained no mutations. Expression of the gfp gene within the presence of arabinose inducer was monitored utilizing fluorescence microscopy of colonies and fluorimetry utilizing each entire cells and lysates.

Conclusion: The outcomes demonstrated the lack of C. jejuni to make use of arabinose transporters, that are totally purposeful in E. coli , suggesting a outstanding distinction within the physiology of those micro organism.

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anti-GCNF

YF-PA23769 50 ul
EUR 334
Description: Mouse polyclonal to GCNF

GCNF ELISA KIT|Human

EF009808 96 Tests
EUR 689

Germ Cell Nuclear Factor (GCNF) Antibody

20-abx131389
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Polyclonal NR6A1 / GCNF Antibody (Ligand-binding Domain)

AMM06820G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NR6A1 / GCNF (Ligand-binding Domain). This antibody is tested and proven to work in the following applications:

Recombinant Germ Cell Nuclear Factor (GCNF)

4-RPC509Hu01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
  • EUR 376.00
  • EUR 3544.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Germ Cell Nuclear Factor expressed in: E.coli

pGL4.19-TA-GCNF response element Plasmid

PVTB00989-2a 2 ug
EUR 356

GCNF (anti human nuclear recepter antibody, clone# H7921)

PP-H7921-00 0.1mg/100uL
EUR 623
Description: The GCNF (anti human nuclear recepter antibody, clone# H7921) is available in Europe and for worldwide shipping via Gentaur.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human)

4-PAC509Hu01
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF)

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), APC

4-PAC509Hu01-APC
  • EUR 345.00
  • EUR 3275.00
  • EUR 912.00
  • EUR 440.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with APC.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), Biotinylated

4-PAC509Hu01-Biotin
  • EUR 311.00
  • EUR 2460.00
  • EUR 727.00
  • EUR 381.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with Biotin.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), Cy3

4-PAC509Hu01-Cy3
  • EUR 419.00
  • EUR 4325.00
  • EUR 1175.00
  • EUR 545.00
  • EUR 251.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with Cy3.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), FITC

4-PAC509Hu01-FITC
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with FITC.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), HRP

4-PAC509Hu01-HRP
  • EUR 316.00
  • EUR 2855.00
  • EUR 807.00
  • EUR 398.00
  • EUR 206.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with HRP.

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), PE

4-PAC509Hu01-PE
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with PE.

Human Germ Cell Nuclear Factor (GCNF) Protein

20-abx650014
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), APC-Cy7

4-PAC509Hu01-APC-Cy7
  • EUR 571.00
  • EUR 6430.00
  • EUR 1705.00
  • EUR 760.00
  • EUR 319.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with APC-Cy7.

H2B Antibody Antibody

AF4659 200ul
EUR 376
Description: H2B Antibody Antibody detects endogenous levels of H2B.

CD11b Antibody Antibody

ABD2911 100 ug
EUR 438

anti- Antibody^Polyclonal antibody control antibody

LSMab09882 100 ug
EUR 438

Antibody

A1360-500 Ask for price

Ly1 Antibody Reactive (LYAR) Antibody

20-abx123734
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Anti-Glycolipid Antibody (AGA) Antibody

abx036399-100ug 100 ug
EUR 391

Ly1 Antibody Reactive (LYAR) Antibody

20-abx014333
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Ly1 Antibody Reactive (LYAR) Antibody

20-abx008109
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Anti-Glycolipid Antibody (AGA) Antibody

20-abx004855
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Ly1 Antibody Reactive (LYAR) Antibody

abx033330-400ul 400 ul
EUR 523

Ly1 Antibody Reactive (LYAR) Antibody

abx033330-80l 80 µl
EUR 286

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319900
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319901
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319905
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody

20-abx319913
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ly1 Antibody Reactive (LYAR) Antibody

20-abx311665
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycolipid Antibody (AGA) Antibody

abx230204-100ug 100 ug
EUR 481

Ly1 Antibody Reactive (LYAR) Antibody

abx234901-100ug 100 ug
EUR 551

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 277
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
EUR 277

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 277

Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody

STJ28365 100 µl
EUR 277

Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Ly1 Antibody Reactive (LYAR) Antibody

20-abx324434
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
EUR 277
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

STJ118549 100 µl
EUR 277

Anti-Anti-MARCH6 Antibody antibody

STJ118550 100 µl
EUR 277

Anti-Anti-MARCH7 Antibody antibody

STJ118752 100 µl
EUR 277

Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
EUR 277

Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ114081 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

STJ114828 100 µl
EUR 277

Ly1 Antibody Reactive Homolog (LYAR) Antibody

20-abx103034
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Ly1 Antibody Reactive Homolog (LYAR) Antibody

20-abx103035
  • EUR 467.00
  • EUR 133.00
  • EUR 1344.00
  • EUR 634.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Ly1 Antibody Reactive Homolog (LYAR) Antibody

20-abx103036
  • EUR 481.00
  • EUR 133.00
  • EUR 1414.00
  • EUR 662.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Hepatitis C Virus Antibody (HCV) Antibody

abx023924-1mg 1 mg
EUR 1205

Anti Deoxyribonucleic Acid Antibody (DNA) Antibody

abx411057-50ug 50 ug
EUR 592

Anti-Glycoprotein Antibody (GP) Antibody (HRP)

20-abx319902
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (FITC)

20-abx319903
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

20-abx319904
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (HRP)

20-abx319906
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (FITC)

20-abx319907
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

20-abx319908
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (HRP)

20-abx319914
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (FITC)

20-abx319915
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

20-abx319916
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (HRP)

20-abx319929
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (FITC)

20-abx319930
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein Antibody (GP) Antibody (Biotin)

20-abx319931
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ly1 Antibody Reactive (LYAR) Antibody (HRP)

20-abx311666
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ly1 Antibody Reactive (LYAR) Antibody (FITC)

20-abx311667
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ly1 Antibody Reactive (LYAR) Antibody (Biotin)

20-abx311668
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-Glycoprotein 210 Antibody (gp210) Antibody

abx233571-100ug 100 ug
EUR 481

Antibody for Human alpha Tubulin Antibody

SPC-692D 0.1mg
EUR 354
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is unconjugated.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A390 0.1mg
EUR 401
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 390.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A488 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 488.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A565 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 565.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A594 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 594.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A633 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 633.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A655 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 655.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A680 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 680.

Antibody for Human alpha Tubulin Antibody

SPC-692D-A700 0.1mg
EUR 400
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 700.

Antibody for Human alpha Tubulin Antibody

SPC-692D-ALP 0.1mg
EUR 394
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Alkaline Phosphatase.

Antibody for Human alpha Tubulin Antibody

SPC-692D-APC 0.1mg
EUR 399
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to APC .

Antibody for Human alpha Tubulin Antibody

SPC-692D-APCCY7 0.1mg
EUR 471
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to APC/Cy7.

Genome-Huge Evaluation of Peptidoglycan Recognition Protein Genes in Fig Wasps (Hymenoptera, Chalcidoidea)

  • The innate immunity is a very powerful protection in opposition to pathogen of bugs, and the peptidoglycan recognition proteins (PGRPs) play an essential function within the processes of immune recognition and initiation of Toll, IMD and different sign pathways.
  • In fig wasps, pollinators and non-pollinators current completely different evolutionary histories and existence, though each are carefully related to fig syconia, which can point out their completely different patterns within the evolution of PGRPs.
  • By handbook annotation, we bought all of the PGRP genes of 12 fig wasp species, containing seven pollinators and 5 non-pollinators, and investigated their putative completely different evolutionary patterns.
  • We discovered that the variety of PGRP genes in pollinators was considerably decrease than in non-pollinators, and the variety of catalytic PGRP introduced a declining pattern in pollinators.
  • Extra importantly, PGRP-SA is related to initiating the Toll pathway, in addition to gram-negative bacteria-binding proteins (GNBPs), which have been fully misplaced in pollinators, which led us to invest that the initiation of Toll pathway was less complicated in pollinators than in non-pollinators.
  • We concluded that fig pollinators owned a extra streamlined innate immune recognition system than non-pollinators. Our outcomes present molecular proof for the adaptive evolution of innate immunity in bugs of host specificity.

Comparative genomics of Alexander Fleming’s authentic Penicillium isolate (IMI 15378) reveals sequence divergence of penicillin synthesis genes 

Antibiotics have been derived initially from wild organisms and due to this fact understanding how these compounds evolve amongst completely different lineages would possibly assist with the design of recent antimicrobial medicine. We report the draft genome sequence of Alexander Fleming’s authentic fungal isolate behind the invention of penicillin, now labeled as Penicillium rubens Biourge (1923) (IMI 15378). We examine the construction of the genome and genes concerned in penicillin synthesis with these in two ‘excessive producing’ industrial strains of P. rubens and the carefully associated species P. nalgiovense.

The primary effector genes for producing penicillin G (pcbAB, pcbC and penDE) present amino acid divergence between the Fleming pressure and each industrial strains, whereas a set of regulatory genes are conserved. Homologs of penicillin N effector genes cefD1 and cefD2 have been additionally discovered and the latter displayed amino acid divergence between the Fleming pressure and industrial strains.

The draft assemblies include a number of partial duplications of penicillin-pathway genes in all three P. rubens strains, to differing levels, which we hypothesise could be concerned in regulation of the pathway.

The 2 industrial strains are equivalent in sequence throughout all effector and regulatory genes however differ in duplication of the pcbAB-pcbC-penDE advanced and partial duplication of fragments of regulatory genes. We conclude that evolution within the wild encompassed each sequence adjustments of the effector genes and gene duplication, whereas human-mediated adjustments by mutagenesis and synthetic choice led to duplication of the penicillin pathway genes.

Human Actinin Alpha 3 (ACTN3) ELISA Kit

RDR-ACTN3-Hu-48Tests 48 Tests
EUR 522

Human Actinin Alpha 3 (ACTN3) ELISA Kit

RDR-ACTN3-Hu-96Tests 96 Tests
EUR 724

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-ACTN3 Antibody (monoclonal, 9B5)

M02693 100ug/vial
EUR 334

ACTN3 Antibody

34157-100ul 100ul
EUR 252

ACTN3 Antibody

34157-50ul 50ul
EUR 187

ACTN3 Antibody

1-CSB-PA124802
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

ACTN3 Antibody

1-CSB-PA288460
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000

ACTN3 Antibody

1-CSB-PA000816
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/20000

ACTN3 Antibody

1-CSB-PA001243LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

ACTN3 Antibody

DF8016 200ul
EUR 304
Description: ACTN3 Antibody detects endogenous levels of total ACTN3.

ACTN3 Antibody

ABD7803 100 ug
EUR 438

ACTN3 Antibody

ABD8016 100 ug
EUR 438

ACTN3 antibody

PAab10008 100 ug
EUR 386

ACTN3 antibody

70R-32269 100 ug
EUR 327
Description: Rabbit polyclonal ACTN3 antibody

ACTN3 antibody

70R-49332 100 ul
EUR 244
Description: Purified Polyclonal ACTN3 antibody

Polyclonal ACTN3 Antibody

APR14796G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 . This antibody is tested and proven to work in the following applications:

ACTN2 / ACTN3 Antibody

20-abx329446
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

ACTN2 / ACTN3 Antibody

abx332374-100ul 100 ul
EUR 425

ACTN2/ACTN3 Antibody

CSB-PA960396-
EUR 335
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

ACTN2/ACTN3 Antibody

CSB-PA960396-100ul 100ul
EUR 316
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

ACTN2/ACTN3 Antibody

1-CSB-PA000815
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ACTN2/ACTN3. Recognizes ACTN2/ACTN3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000

ACTN3 Conjugated Antibody

C34157 100ul
EUR 397

ACTN3 siRNA

20-abx906644
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

ACTN3 siRNA

20-abx906645
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

Polyclonal ACTN3 Antibody (Center)

APR14798G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 (Center). This antibody is tested and proven to work in the following applications:

ACTN3 Antibody, HRP conjugated

1-CSB-PA001243LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ACTN3 Antibody, FITC conjugated

1-CSB-PA001243LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ACTN3 Antibody, Biotin conjugated

1-CSB-PA001243LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ACTN3. Recognizes ACTN3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Anti-Human ACTN3 DyLight® 488 conjugated Antibody

A02693-Dyl488 100ug/vial
EUR 344

Anti-Human ACTN3 DyLight® 550 conjugated Antibody

A02693-Dyl550 100ug/vial
EUR 344

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

ACTN3 Blocking Peptide

20-abx062207
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

ACTN3 Rabbit pAb

A12797-100ul 100 ul
EUR 308

ACTN3 Rabbit pAb

A12797-200ul 200 ul
EUR 459

ACTN3 Rabbit pAb

A12797-20ul 20 ul
EUR 183

ACTN3 Rabbit pAb

A12797-50ul 50 ul
EUR 223

ACTN3 Rabbit pAb

A12864-100ul 100 ul
EUR 308

ACTN3 Rabbit pAb

A12864-200ul 200 ul
EUR 459

ACTN3 Rabbit pAb

A12864-20ul 20 ul
EUR 183

ACTN3 Rabbit pAb

A12864-50ul 50 ul
EUR 223

ACTN3 Blocking Peptide

DF8016-BP 1mg
EUR 195

Actinin Alpha 3 (ACTN3) Antibody

20-abx130639
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Actinin Alpha 3 (ACTN3) Antibody

abx038105-100ug 100 ug
EUR 391

Actinin Alpha 3 (ACTN3) Antibody

20-abx009624
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Actinin Alpha 3 (ACTN3) Antibody

20-abx013892
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Actinin Alpha 3 (ACTN3) Antibody

abx026732-400ul 400 ul
EUR 523

Actinin Alpha 3 (ACTN3) Antibody

abx026732-80l 80 µl
EUR 286

Actinin Alpha 3 (ACTN3) Antibody

abx230122-100ug 100 ug
EUR 509

Alpha Actinin 3 (ACTN3) Antibody

20-abx213418
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Alpha Actinin 3 (ACTN3) Antibody

20-abx213419
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Polyclonal ACTN3 Antibody (aa1-50)

APR14797G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ACTN3 (aa1-50). This antibody is tested and proven to work in the following applications:

ACTN1 / ACTN2 / ACTN3 / ACTN4 Antibody

abx332562-100ul 100 ul
EUR 425

Alpha Actinin 3 (ACTN3) Antibody

20-abx328117
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

ACTN1 / ACTN2 / ACTN3 / ACTN4 Antibody

20-abx328684
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

ACTN1/ACTN2/ACTN3/ACTN4 Antibody

CSB-PA096347-
EUR 335
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

ACTN1/ACTN2/ACTN3/ACTN4 Antibody

CSB-PA096347-100ul 100ul
EUR 316
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

ACTN1/ACTN2/ACTN3/ACTN4 Antibody

1-CSB-PA000814
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ACTN1/ACTN2/ACTN3/ACTN4. Recognizes ACTN1/ACTN2/ACTN3/ACTN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

Mouse ACTN3 ELISA Kit

EMA0390 96Tests
EUR 521

Porcine ACTN3 ELISA Kit

EPA0390 96Tests
EUR 521

Rat ACTN3 ELISA Kit

ERA0390 96Tests
EUR 521

Rabbit ACTN3 ELISA Kit

ERTA0390 96Tests
EUR 521

Anserini ACTN3 ELISA Kit

EAA0390 96Tests
EUR 521

Goat ACTN3 ELISA Kit

EGTA0390 96Tests
EUR 521

Canine ACTN3 ELISA Kit

ECA0390 96Tests
EUR 521

Human ACTN3 ELISA Kit

EHA0390 96Tests
EUR 521

Bovine ACTN3 ELISA Kit

EBA0390 96Tests
EUR 521

Human ACTN3 shRNA Plasmid

20-abx950055
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse ACTN3 shRNA Plasmid

20-abx969019
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

ACTN3 ELISA KIT|Human

EF003149 96 Tests
EUR 689

Human ACTN3 ELISA Kit

ELA-E1104h 96 Tests
EUR 824

Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human)

4-PAB104Hu01
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3)

Guinea Pig ACTN3 ELISA Kit

EGA0390 96Tests
EUR 521

ACTN3 ELISA Kit (Human) (OKEH04449)

OKEH04449 96 Wells
EUR 662
Description: Description of target: This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 4.1 pg/mL

Recombinant Actinin Alpha 3 (ACTN3)

4-RPB104Hu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Actinin Alpha 3 expressed in: E.coli

ACTN3 ELISA Kit (Mouse) (OKEH05687)

OKEH05687 96 Wells
EUR 662
Description: Description of target: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 41 pg/mL

ACTN3 ORF Vector (Human) (pORF)

ORF012141 1.0 ug DNA
EUR 354

Actn3 ORF Vector (Mouse) (pORF)

ORF038023 1.0 ug DNA
EUR 506

Actn3 ORF Vector (Rat) (pORF)

ORF063016 1.0 ug DNA
EUR 506

Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), APC

4-PAB104Hu01-APC
  • EUR 333.00
  • EUR 3113.00
  • EUR 872.00
  • EUR 423.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with APC.

Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), Biotinylated

4-PAB104Hu01-Biotin
  • EUR 303.00
  • EUR 2341.00
  • EUR 697.00
  • EUR 369.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with Biotin.

Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), Cy3

4-PAB104Hu01-Cy3
  • EUR 403.00
  • EUR 4109.00
  • EUR 1121.00
  • EUR 523.00
  • EUR 245.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with Cy3.

Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), FITC

4-PAB104Hu01-FITC
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with FITC.

Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), HRP

4-PAB104Hu01-HRP
  • EUR 305.00
  • EUR 2714.00
  • EUR 772.00
  • EUR 383.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with HRP.

Actinin Alpha 3 (ACTN3) Polyclonal Antibody (Human), PE

4-PAB104Hu01-PE
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Actinin Alpha 3 (ACTN3). This antibody is labeled with PE.

Actinin Alpha 3 (ACTN3) CLIA Kit

20-abx492418
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Actinin Alpha 3 (ACTN3) ELISA Kit

20-abx156912
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Actinin Alpha 3 (ACTN3) Protein

20-abx167185
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Transposon Insertion within the purL Gene Induces Biofilm Depletion in Escherichia coli ATCC 25922

Present Escherichia coli antibiofilm therapies comprise a mix of antibiotics generally used in opposition to planktonic cells, resulting in therapy failure. A greater understanding of the genes concerned in biofilm formation may facilitate the event of environment friendly and particular new antibiofilm therapies.

A complete of 2578 E. coli mutants have been generated by transposon insertion, of which 536 have been analysed on this research. After sequencing, Tn263 mutant, labeled as low biofilm-former (LF) in contrast to the wild-type (wt) pressure (ATCC 25922), confirmed an interruption within the purL gene, concerned within the de novo purine biosynthesis pathway.

To elucidate the function of purL in biofilm formation, a knockout was generated displaying decreased manufacturing of curli fibres, resulting in an impaired biofilm formation. These situations have been restored by complementation of the pressure or addition of exogenous inosine. Proteomic and transcriptional analyses have been carried out to characterise the variations attributable to purL alterations.

13 proteins have been altered in contrast to wt. The corresponding genes have been analysed by qRT-PCR not solely within the Tn263 and wt, but additionally in medical strains with completely different biofilm exercise. Total, this research means that purL is important for biofilm formation in E. coli and might be thought of as a possible antibiofilm goal.

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